Nano-Glo Luciferase Assay N1120 from Promega

Product Specs
ItemNano-Glo Luciferase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1120
Quantity100ml

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Biochemical Reconstitution of Ca2+-Dependent Exosome Secretion in Permeabilized Mammalian CellsBio ProtocDecember 5, 2023Jordan M. Ngo, Justin K. Williams, Isabelle M. Lehman, Randy SchekmaninhibitorNanoBRETTM Nano-Glo® substrateNano-Glo® Luciferase Assay System (Promega, catalog number: N1120)Nano-Glo® Luciferase assay substrateNano-Glo® Luciferase assay buffer Solutions 1 M HEPES, pH 7.4 (see

(2) Inhibition of SARS-CoV-2 infection in human airway epithelium with a xeno-nucleic acid aptamerRespiratory ResearchNovember 7, 2023Niayesh Razi, Weizhong Li, Maxinne A. Ignacio, Jeffrey M. Loube, Eva L. Agostino, Xiaoping Zhu, Margaret A. Scull, Jeffrey J. DeStefanoluciferase activity in each well was quantified using the Nano-Glo® Luciferase Assay System (Cat No. N1120; Promega) according to the manufacturer’s protocol and a Berthold Centro XS3 LB 960 plate reader. Relative

(3) Gα Protein Signaling Bias at Serotonin 1A ReceptorMolecular PharmacologyNovember 1, 2023Rana Alabdali, Luca Franchini, Cesare Orlandicells were plated in 96-well white microplates (Greiner Bio-One). The nanoluc substrate furimazine (N1120) was purchased from Promega and used according to the manufacturer's instructions. BRET measurements

(4) Creating Designer Engineered Extracellular Vesicles for Diverse Ligand Display, Target Recognition, and Controlled Protein Loading and DeliveryAdvanced ScienceOctober 22, 2023Alena Ivanova, Lukas Badertscher, Gwen O'Driscoll, Joakim Bergman, Euan Gordon, Anders Gunnarsson, Camilla Johansson, Michael J. Munson, Cristiana Spinelli, Sara Torstensson, et al.The presence of NanoLuc luciferase in cells was measured using the Nano‐Glo Luciferase assay system (N1120, Promega) following the manufacturer´s protocol. Luminescence was measured using the PHERAstar FSX instrument

(5) Novel Antiviral Molecules against Ebola Virus InfectionInternational Journal of Molecular SciencesSeptember 30, 2023Mila Collados Rodríguez, Patrick Maillard, Alexandra Journeaux, Anastassia V. Komarova, Valérie Najburg, Raul-Yusef Sanchez David, Olivier Helynck, Mingzhe Guo, Jin Zhong, Sylvain Baize, et al.were incubated in the presence of 40 μL of Nano-Glo luciferase reagent (Promega, Southampton, UK; #N1120). Luciferase enzymatic activity was measured with a Centro XS LB960 luminometer (Berthold, Bad Wildbad,

(6) Anthracyclines inhibit SARS-CoV-2 infectionVirus researchSeptember 1, 2023Wang Z, Pan Q, Ma L, Zhao J, McIntosh F, Liu Z, Ding S, Lin R, Cen S, Finzi A, et al.luciferase activity in the infected cells was measured using Nano-Glo Luciferase Assay System (Promega, cat. N1120). Inhibition of SARS-CoV-2 variants RIM-1, SA and BA.1 was tested in 24-well plates. Calu-3 cells were

(7) Bioluminescence Assay of Lysine Deacylase Sirtuin ActivitybioRxivAugust 18, 2023Alexandria N. Van Scoyk, Orlando Antelope, Anca Franzini, Donald E. Ayer, Randall T. Peterson, Anthony D. Pomicter, Shawn C. Owen, Michael W. Deiningerat the 1-cell stage. For preliminary testing of endurazine (Promega, N2570) and furimazine (Promega, N1120), 5 μL reactions were made by adding 4 μL LgBiT (Promega, N1120), 0.5 μL 100 μM p86-R4 (Vivitide) or

(8) Targeting the Clear Cell Sarcoma Oncogenic Driver Fusion Gene EWSR1::ATF1 by HDAC InhibitionCancer Research CommunicationsJuly 3, 2023Hirokazu Mae, Hidetatsu Outani, Yoshinori Imura, Ryota Chijimatsu, Akitomo Inoue, Yuki Kotani, Naohiro Yasuda, Sho Nakai, Takaaki Nakai, Satoshi Takenaka, et al.assayed after vorinostat or vehicle treatment for 6 hours using the Nano-Glo Luciferase Assay System (N1120; Promega) with the GloMax Navigator System (Promega) according to the manufacturer's instructions. A

(9) CaBLAM! A high-contrast bioluminescent Ca 2+ indicator derived from an engineered Oplophorus gracilirostris luciferasebioRxivJuly 1, 2023Gerard G. Lambert, Emmanuel L. Crespo, Jeremy Murphy, Daniela Boassa, Selena Luong, Junru Hu, Brittany Sprecher, Maya O. Tree, Richard Orcutt, Daniel Heydari, et al.solution was purchased from Promega as a component of the Nano-Glo® Luciferase Assay System (cat# N1110 or N1120). Because Promega does not disclose the Fz concentration in their stock solution, we hereafter describe

(10) GPCR Binding and JNK3 Activation by Arrestin-3 Have Different Structural RequirementsCellsJune 6, 2023Chen Zheng, Liana D. Weinstein, Kevin K. Nguyen, Abhijeet Grewal, Eugenia V. Gurevich, Vsevolod V. Gurevich(16 h) in culture medium without phenol red. At 48 h post-transfection, luciferase substrate nanoGlo (N1120; Promega, Fitchburg, WI, USA) was added according to the manufacturer’s instructions, and the total

(11) Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availabilityeLifeJune 2, 2023Anil Kumar Vijjamarri, Xiao Niu, Matthew D Vandermeulen, Chisom Onu, Fan Zhang, Hongfang Qiu, Neha Gupta, Swati Gaikwad, Miriam L Greenberg, Paul J Cullen, et al.beads, and clarified by centrifugation at 13,000 × g for 30 min at 4°C. Nano-Glo substrate (Promega, N1120) was diluted 1:50 with the supplied lysis buffer and mixed with 10 µl of WCE in a white 96-well plate.

(12) Translation re-initiation after uORFs does not fully protect mRNAs from nonsense-mediated decayRNAJune 1, 2023Paul J. Russell, Jacob A. Slivka, Elaina P. Boyle, Arthur H.M. Burghes, Michael G. KearseµL of diluted reaction was mixed with 25 µL of prepared Nano-Glo Luciferase Assay System (Promega # N1120) for 5 min in the dark on an orbital shaker. Luminescence was measured using a Promega GloMax Discover Microplate Reader

(13) Generation of a SARS-CoV-2 Reverse Genetics System and Novel Human Lung Cell Lines That Exhibit High Virus-Induced CytopathologyVirusesMay 30, 2023Khan JQ, Rohamare M, Rajamanickam K, Bhanumathy KK, Lew J, Kumar A, Falzarano D, Vizeacoumar FJ, Wilson JA.performed. Reporter luciferase assays: The Nano-Glo® luciferase assay system (Promega, Madison, WI, USA, #N1120) was used to assess NLuc expression by SARS-CoV-2 NLucFL, as per the manufacturer’s protocol. Briefly,

(14) Insight into the Mode of Action of 8-Hydroxyquinoline-Based Blockers on the Histamine Receptor 2Biosensors (Basel)May 23, 2023Amisha Patel, Paola L. Marquez-Gomez, Lily R. Torp, Lily Gao, Pamela Peralta-YahyaMethods Materials. Luciferase expression was assayed using the NanoGlo® Luciferase Assay System (Promega N1120). Histamine dihydrochloride (Sigma H7250), chlorquinaldol (Selleck S4192), and chloroxine (Selleck S1839)

(15) Plasma membrane preassociation drives β-arrestin coupling to receptors and activationCellMay 6, 2023Jak Grimes, Zsombor Koszegi, Yann Lanoiselée, Tamara Miljus, Shannon L. O’Brien, Tomasz M. Stepniewski, Brian Medel-Lacruz, Mithu Baidya, Maria Makarova, Ravi Mistry, et al.corresponding to the C-terminal region of the V2R (V2Rpp),Shukla et al.7,N/A Furimazine,Promega,Cat#N1120 Isoproterenol hydrocholoride,Tocris,Cat#1747 Arginine-vasopressin,Sigma,Cat#V9879 Parathyroid hormone

(16) Genetically engineered pair of cells for serological testing and its application for SARS‐CoV‐2Bioengineering & Translational MedicineMay 1, 2023Marvin A. Ssemadaali, Juan Arredondo, Elise A. Buser, Sherri Newmyer, Harikrishnan Radhakrishnan, Harold S. Javitz, Satya Dandekar, Parijat Bhatnagarwere used for characterization experiments of the DxCell‐Complex. Nano‐Glo assay kit (Promega, Cat #N1120) was used to assess expression of the Nluc protein. 4.2 Source of COVID‐19 patient sera samples For

(17) The SpACE-CCM: A facile and versatile cell culture medium-based biosensor for detection of SARS-CoV-2 spike-ACE2 interactionBiosensors & bioelectronicsMay 1, 2023Ham Y, Cho NC, Kim D, Kim JH, Jo MJ, Jeong MS, Pak BY, Lee S, Lee MK, Chi SW, et al.μl of cell culture medium were analyzed by directly adding the Nano-Glo Live reagent (Promega, Cat.#N1120) to each well as per the manufacturer’s protocol. The Nano-Glo Live reagent is a non-lytic detection

(18) Dissecting the molecular basis for the modulation of neurotransmitter GPCR signaling by GINIPbioRxivApril 25, 2023Alex Luebbers, Myles Zhou, Stephen J Eyles, Mikel Garcia-MarcosPerkinElmer Life Sciences, 6005290) and mixed with the nanoluciferase substrate Nano-Glo (Promega, N1120, final dilution 1:200) before measuring luminescence. Luminescence signals at 450 ± 40 and 535 ± 15

(19) Cell-of-origin–specific proteomics of extracellular vesiclesPNAS NexusApril 3, 2023Sebastian Kehrloesser, Oliver Cast, Thomas S Elliott, Russell J Ernst, Anne C Machel, Jia-Xuan Chen, Jason W Chin, Martin L Miller7.2, 150 mm NaCl, 0.05% Tween-20, and EV recovery was measured by NanoGlow luciferase assay (Promega N1120) and recovery normalized to the respective input signal. For the analysis of luciferase recovery from

(20) Large DNA fragment knock-in and sequential gene editing in Plasmodium falciparum: a preliminary study using suicide-rescue-based CRISPR/Cas9 systemMolecular and Cellular BiochemistryApril 1, 2023Junnan Lu, Ying Tong, Rui Dong, Yijun Yang, Wen Hu, Minghong Zhang, Quan Liu, Siting Zhao, John H. Adams, Li Qin, et al.expression of Nanoluc luciferase was confirmed using the Nano-Glo® Luciferase Assay System (Code No: N1120; Promega Corporation). Parasites were lysed using distilled water, and an equal volume of diluted substrate

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3/16/2017NanoLuciferase Assay System Nina FlerinOur laboratory investigates different ways to improve immune responses against HIV.. We developed different mouse models to study the efficacy of therapeutic candidates against HIV. Some of these mice... Read Review
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3/2/2017Nano-Glo® Luciferase Assay System Review Lana JagannathanOur lab uses HIV molecular clones that express Nano luciferase reporter. This reporter is very stable and allows quatification of low HIV infection, because it provides a very sensitive reading. We ... Read Review

Nano-Glo Luciferase Assay

Nano-Glo Luciferase Assay from Promega

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Nano-Glo Luciferase Assay from Promega

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